To produce virus-free plants, a simple and original protocol was established by combining several techniques: repeated shoot meristem excision before and during in vitro culture and thermotherapy applied to bulblets in vitro. Lily symptomless virus LSV is a major virus that decreases plant growth vigor and the quality of cut flowers, yet infected plants show no distinct symptoms. Stock bulbs of pollenless Asiatic hybrid lily L. Shoot meristems were excised and micropropagated. Leaf tissues from bulblets formed before or postheat treatments were analyzed either by enzyme-linked immunosorbent assay or by reverse transcriptase-polymerase chain reaction.
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